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Single Domain Antibody Services

In terms of the extensive experience in antibody research, Creative Biolabs is proud to offer our clients a series of single domain antibody services with the best quality and most competitive price.

What is Single Domain Antibody?

Single domain antibody, also known as domain antibody, VHH, VNAR or sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in conventional Fab region. The first single domain antibody was engineered from the VHH domain of heavy-chain antibody identified in camelids (e.g. dromedaries, camels, llamas, and alpacas). And the VNAR domain of cartilaginous fishes (e.g. shark) heavy-chain antibody (known as IgNAR, immunoglobulin new antigen receptor) is another qualified source for sdAb development. An alternative approach is to split the dimeric variable domains from normal IgG of humans or mice into monomers by camelizing a few critical residues.

Similar to a conventional antibody, single domain antibody is able to bind selectively to a specific antigen. In terms of only 12-15 kDa molecular weight, which is much smaller than either full length antibody (150-160 kDa) or other antibody fragments (Fab ~50 kDa, scFv ~25 kDa), single domain antibody takes great advantages of stability and penetrability, which are essential to the development of several antibody drugs or diagnostic tools.

Advantages of Single Domain Antibody

High stability to function and exist within extreme conditions
Outstanding penetrability which is able to cross the blood-brain barrier
Recognize novel epitopes that regular size antibodies cannot
Improve bioavailability for pharmaceutical applications
Great potential in downstream engineering (bispecific antibody, humanization)
Can be expressed in both eukaryotic and prokaryotic systems

Creative Biolabs is a well-recognized leader in the field of single domain antibody. Based on our advanced phage display technology, our scientists are specialized in the production and discovery of specific single domain antibodies. According to our client’s demands, we can immunize camel, llama, alpaca or even shark as repertoire source to develop single domain antibodies against interested targets (antigens). Our advanced technology allows various targets to be employed in immunization, including large protein molecules, membrane proteins, haptens and other challenging targets. As an alternative, Creative Biolabs can also screen our pre-made HuSdL® Human Single Domain Antibody Library to isolate high-potency camelized human single domain antibodies. In addition to our sdAb related services, Creative Biolabs also provides a comprehensive list of recombinant single domain antibody products to meet our customers’ requirements.

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publié le mardi 14 mai à 07:23

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